Transcriptomics-based points of departure for Daphnia magna exposed to 18 per- and polyfluoroalkyl substances.

Daphnia magna were exposed to multiple concentrations of 22 different PFAS for 24 h, in 96-well plate format. Following exposure, whole body RNA was extracted and extracts, each representing five exposed individuals, were subjected to RNA sequencing. Following analytical measurements to verify PFAS exposure concentrations, and quality control on processed cDNA libraries for sequencing, concentration-response modeling was applied to the data sets for 18 of the tested compounds, and the concentration at which a concerted molecular response occurred (transcriptomic point of departure; tPOD) was calculated. This file provides the processed and normalized count matrices for each of the 18 PFAS evaluated along with exported results from BMDExpress 3.0 (tab delimited .txt files). Raw data were submitted to the National Center for Biotechnology Information Sequence Read Archive (Bioproject PRJNA1018977) and Gene Expression Omnibus (GEO Accession GSE245268). Sample meta data copied here.