In vitro estrogenic activity of perfluorinated diols of different chain lengths

Per- and polyfluoroalkyl substances (PFAS) are a diverse class of synthetic fluorinated chemicals that are currently ubiquitous in the environment due to their diverse usage in both consumer and industrial products. To help prioritize data poor PFAS, previous work evaluated 142 PFAS using an in vitro screening platform. To further evaluate effectiveness of the assays in identifying PFAS capable of inducing estrogenic responses in fish our lab group conducted in vivo exposures using fathead minnows (Pimephales promelas). 1H,1H,8H,8H-perfluorooctane-1,8-diol [FC8-diol], 1H,1H,10H,10H-perfluorodecane-1,10-diol [FC10-diol], and 1H,1H,8H,8H-perfluoro-3,6-dioxaoctane-1,8-diol [FC8-DOD] were found to be estrogenic in fathead minnows. The data stored here builds off these studies and was aimed to examine the ability of similar PFAS, primarily PFAS with the same structure but different chain lengths, to induce estrogenic responses in vitro. One PFAS with a high similarity score to FC10-diol (the most potent of the tested PFAS) but different structure was also included. Presented here are the data from Indigo ER bioassays along with data from a rainbow trout liver slice assay which measured estrogen receptor-regulated gene expression. Data sheets include the raw data from the plate reader (presented in blue under luminescence) and resulting data (E2 equivalence) and figures from PRISM analysis. Data sheets are labeled by date and chemicals tested. The second folder includes data sheets from rainbow trout liver slice assays. This folder includes RNA concentrations and purities from all exposures, normalized RNA used for qPCR, plate layouts, raw data from qPCR runs, and preliminary figures from qPCR.